製品の概要

  • 製品名Anti-FGF9 antibody
    FGF9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FGF9
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
    交差が予測される動物種: Rat, Chicken, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF9.

    (Peptide available as ab86940.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Stomach Tumour Tissue; HEK293 Whole Cell; Mouse Brain Tissue; Mouse Kidney Tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

  • 機能May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • 組織特異性Glial cells.
  • 関連疾患Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 翻訳後修飾Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FGF 9 antibody
    • FGF-9 antibody
    • FGF9 antibody
    • FGF9_HUMAN antibody
    • Fibroblast growth factor 9 antibody
    • GAF antibody
    • Glia Activating Factor antibody
    • Glia-activating factor antibody
    • HBFG 9 antibody
    • HBFG9 antibody
    • HBGF-9 antibody
    • Heparin-binding growth factor 9 antibody
    • MGC119914 antibody
    • MGC119915 antibody
    • SYNS3 antibody
    see all

Anti-FGF9 antibody 画像

  • ICC/IF image of ab71395 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71395, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, hek293 and MCF7 cells at 5µg/ml.
  • IHC image of FGF9 staining in FFPE human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71395, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-FGF9 antibody (ab71395) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678)
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human FGF9 peptide (ab86940) at 1 µg/ml
    Lane 5 : Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) with Human FGF9 peptide (ab86940) at 1 µg/ml
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human FGF9 peptide (ab86940) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 23 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    The peptide blocking data above shows that ab71395 detects a protein of approximately 50 kDa, thought to be FGF9. It is possible that this band is significantly higher than expected due to modifications at potential glycosylation sites.
  • All lanes : Anti-FGF9 antibody (ab71395) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ab71395 staining FGF9 in monkey fetal lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-FGF9 antibody (ab71395) 使用論文

This product has been referenced in:
  • Chung CL  et al. Association of aberrant expression of sex-determining gene fibroblast growth factor 9 with Sertoli cell-only syndrome. Fertil Steril 100:1547-54.e1-4 (2013). Human . Read more (PubMed: 24011613) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate PH 6.0
Specification lungs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Monkey Tissue sections (Fetal lung)
Specification Fetal lung
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 30 2014

Vielen Dank für Ihre Anfrage.

Leider haben wir nicht das passende Blockpeptid für den ab106245 Anti-FGF2 antibody in unserem Katalog, dafür aber das aktive, rekommbinate Protein ab129033:

Click here (or use the following: http://w...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"