Anti-FGF21 抗体 [EPR8314(2)] (ab171941)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8314(2)] to FGF21
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FGF21 antibody [EPR8314(2)]
FGF21 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8314(2)] to FGF21 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.
Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327). For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FGF21 aa 1-100. The exact sequence is proprietary.
Database link: Q9NSA1 -
ポジティブ・コントロール
- Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. WB: Human fetal liver lysate, mouse spleen, rat spleen. IHC-P: Human stomach
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8314(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171941の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 22 kDa. |
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 26291 Human
- Entrez Gene: 56636 Mouse
- Entrez Gene: 170580 Rat
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- SwissProt: Q9JJN1 Mouse
- Unigene: 283015 Human
- Unigene: 143736 Mouse
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別名
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
画像
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All lanes : Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution
Lane 1 : Human Liver cell lysate
Lane 2 : Mouse Spleen cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-FGF21 antibody [EPR8314(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab171941 was shown to bind specifically to FGF21. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution
Lane 1 : Wild-type HeLa Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 2 : FGF21 knockout HeLa Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 3 : Wild-type HeLa Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 20 µg
Lane 4 : FGF21 knockout HeLa Vehicle Control BFA (0 ug/mL, 6 h) cell lysate at 20 µg
Lane 5 : HepG2 Treated BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 6 : HepG2 cell lysate at 20 µg
Lane 7 : Empty
Lane 8 : Human Liver cell lysate at 10 µg
Lane 9 : Mouse Spleen cell lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-FGF21 antibody [EPR8314(2)] staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab171941 was shown to bind specifically to FGF21. A band was observed at 21 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in FGF21 knockout cell line ab265974 (knockout cell lysate ab256915). To generate this image, wild-type and FGF21 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development and with high-sensitivity chemiluminescence substrate and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/1000 dilution (purified) + Mouse spleen tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Rat spleen tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution (purified) + Human fetal liver tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human stomach with purified ab171941 at a working dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (64)
ab171941 は 64 報の論文で使用されています。
- Chen X et al. KLF4 downregulates FGF21 to activate inflammatory injury and oxidative stress of LPS-induced ATDC5 cells via SIRT1/NF-?B/p53 signaling. Mol Med Rep 25:N/A (2022). WB ; Human . PubMed: 35293599
- Zhang Y et al. Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism. Cell Rep Med 3:100498 (2022). PubMed: 35106510
- Higgins CB et al. SIRT1 selectively exerts the metabolic protective effects of hepatocyte nicotinamide phosphoribosyltransferase. Nat Commun 13:1074 (2022). PubMed: 35228549
- Zhang Y et al. Epigallocatechin-3-gallate ameliorates hepatic damages by relieve FGF21 resistance and promotion of FGF21-AMPK pathway in mice fed a high fat diet. Diabetol Metab Syndr 14:53 (2022). PubMed: 35418153
- Kawakami R et al. Ketone body and FGF21 coordinately regulate fasting-induced oxidative stress response in the heart. Sci Rep 12:7338 (2022). PubMed: 35513524