製品の概要

  • 製品名Anti-FGF21 antibody
    FGF21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FGF21
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human FGF21.

    (Peptide available as ab66563.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein (Human)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    Anti-FGF21 antibody 画像

    • Anti-FGF21 antibody (ab66564) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 22 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
    • IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-FGF21 antibody (ab66564) 使用論文

    This product has been referenced in:
    • Yang C  et al. Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress. BMC Gastroenterol 13:67 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23590285) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"