製品の概要

  • 製品名Anti-FGF2 antibody [EP1735]
    FGF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1735] to FGF2
  • アプリケーション適用あり: WB, IP, Flow Cyt, Sandwich ELISAmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human FGF basic.

  • ポジティブ・コントロール
    • K562 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AS24] to FGF2 (ab17279). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
    • 組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
    • 配列類似性Belongs to the heparin-binding growth factors family.
    • 翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
      Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
    • 細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    Anti-FGF2 antibody [EP1735] 画像

    プロトコール

    Anti-FGF2 antibody [EP1735] (ab92337) 使用論文

    ab92337 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (HepG2)
    Specification HepG2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Dec 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"