製品の概要

  • 製品名Anti-FGF2 antibody [AS24]
    FGF2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AS24] to FGF2
  • 特異性Recognises human basic FGF, does not recognise human acidic FGF or a panel of other human cytokines.
  • アプリケーション適用あり: IHC-Fr, Sandwich ELISA, ELISAmore details
  • 種交差性
    交差種: Human

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab17279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4°C, plus 0.1% saponin for 20minutes at room temperature.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP1735] to FGF2 (ab92337). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit monoclonal [EP1735] to FGF basic (ab92337) as Detection.
ELISA Use at an assay dependent concentration. This antibody can be used as a coating antibody at 10 µg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA.

ターゲット情報

  • 機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody [AS24] 画像

プロトコール

Anti-FGF2 antibody [AS24] (ab17279) 使用論文

This product has been referenced in:
  • Chan WK  et al. 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS One 10:e0130147 (2015). Read more (PubMed: 26075383) »

See 1 Publication for this product

Product Wall

Thank you for your email. At this point I can offer a free of charge replacement vial of ab17270 or a refund for your customer. Please let me know how the customer would like to proceed.

Thank you for your enquiry. At this point I would like to make the following suggestions to help your customer out with this antibody. In addition to using H2O2 to suppress endogenous peroxidase activity, make sure to block in 10% NS with 1% BSA in...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"