製品の概要

  • 製品名Anti-FGF2 antibody
    FGF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FGF2
  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide:

    GFFLRIHPDGRVDGVREKSDPHIK

    , corresponding to amino acids 40-63 of Human FGF basic.

  • ポジティブ・コントロール
    • Fetal liver or kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 22302773
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 18 kDa.

ターゲット情報

  • 機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody 画像

  • ab16828 at 5ug/ml staining human B lineage lymphoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 1 hour. Bound antibody was then detected using a biotinylated horse anti-rabbit and mouse IgG antibody.

    This image is courtesy of an Abreview submitted by Francisca Wu on 15 March 2006.

    See Abreview

Anti-FGF2 antibody (ab16828) 使用論文

This product has been referenced in:
  • Laquer VT  et al. Immunohistochemistry of angiogenesis mediators before and after pulsed dye laser treatment of angiomas. Lasers Surg Med 44:205-10 (2012). IHC-Fr ; Human . Read more (PubMed: 22302773) »
  • Guimei M  et al. Gremlin in the pathogenesis of hepatocellular carcinoma complicating chronic hepatitis C: an immunohistochemical and PCR study of human liver biopsies. BMC Res Notes 5:390 (2012). IHC-P ; Human . Read more (PubMed: 22839096) »

See all 6 Publications for this product

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Thank you for contacting us. There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications. Main cardiac proteins are as follows; - alpha actin; alpha s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.1
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
Username

Ms. Elizabeth Chlipala

Verified customer

投稿 Oct 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (tendon)
Specification tendon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrat puffer ph 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Feb 06 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (b-lineage lymphoma)
Specification b-lineage lymphoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Francisca Wu

Verified customer

投稿 Mar 15 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"