製品の概要
-
製品名Anti-FGF2 antibody
FGF2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to FGF2
-
由来種Rabbit
-
アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
-
種交差性交差種: Human
-
免疫原
Synthetic peptide:
GFFLRIHPDGRVDGVREKSDPHIK
, corresponding to amino acids 40-63 of Human FGF basic. -
ポジティブ･コントロール
- IHC-P: Human fetal liver or kidney tissue; Human hepatitis, cirrhosis and HCC liver and pancreas tissues;.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
バッファーPreservative: None
Constituents: 50% Glycerol -
Concentration information loading... -
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab16828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22302773 | |
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 18 kDa. |
ターゲット情報
-
機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
-
組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
-
配列類似性Belongs to the heparin-binding growth factors family.
-
翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
- Information by UniProt
-
参照データベース
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
-
別名
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody (ab16828)Formaldehyde-fixed, paraffin-embedded human pancreas tissue stained for FGF using ab16828 at 1/50 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody (ab16828)Paraffin-embedded human liver tissue stained for FGF using ab16828. A case of chronic hepatitis showing a few positive hepatocytes in (a) periportal areas (×200), (b) the pericentral zone (×400) and (c) intermediate hepatocytes (arrow) (×200). In HCC, (d) positivity was also detected in HCC tumor cells (×200).
PMID: 22839096
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody (ab16828)ab16828 at 5ug/ml staining human B lineage lymphoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 1 hour. Bound antibody was then detected using a biotinylated horse anti-rabbit and mouse IgG antibody.
This image is courtesy of an Abreview submitted by Francisca Wu on 15 March 2006.
参考文献
This product has been referenced in:
- Zhang F et al. Ligand Activation of PPAR? by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1a. Theranostics 8:610-626 (2018). Read more (PubMed: 29344293) »
- Chen Y et al. Degradation of Mg-6Zn alloy stents does not influence the healing of the common bile duct in vivo. Exp Ther Med 13:2651-2656 (2017). Read more (PubMed: 28587326) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"