製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FGF18
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF18.

    (Peptide available as ab91611.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human skin and small intestine tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能
    Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.
  • 配列類似性
    Belongs to the heparin-binding growth factors family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FGF 18 antibody
    • FGF-18 antibody
    • Fgf18 antibody
    • FGF18_HUMAN antibody
    • FGFI antibody
    • Fibroblast growth factor 18 antibody
    • zFGF5 antibody
    see all

画像

  • All lanes : Anti-FGF18 antibody (ab63037) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes


    The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human Fibroblast growth factor 18 precursor (FGF18). Furthermore FGF18 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

プロトコール

参考文献

ab63037 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you very much for your interest in Abcam FGF18 antibodies.

The immunogen of http://www.abcam.com/ab63037 has 82% homology with the Chicken protein. However, immunogens of both http://www.abcam.com/ab86571 and http://www.abcam.com/ab13390...

Read More

Thank you for your enquiry regarding ab63037. The immunogen used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF18. Unfortunately, we are not able to release the exact im...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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