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Our Abpromise guarantee covers the use of ab9990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml. (using 100µl/well antibody solution). Allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.|
|Neutralising||Use a concentration of 0.02 - 0.035 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/ml).|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [MM0284-5H27] to FGF17 (ab89270). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab89270 as Capture.|
ab9990 has not yet been referenced specifically in any publications.