The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 17 kDa.
1/10 - 1/50.
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
Belongs to the heparin-binding growth factors family.
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
Immunohistochemical analysis of FGF1 in formalin-fixed, paraffin-embedded human colon carcinoma tissue using ab75928, at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.