製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to FGF1
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 46-155 of Human FGF1

  • ポジティブ・コントロール
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
ELISA
  • 追加情報
    ELISA: Use at a dilution of 1/250 for 8 hrs.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
    • 配列類似性
      Belongs to the heparin-binding growth factors family.
    • 細胞内局在
      Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acidic fibroblast growth factor antibody
      • aFGF antibody
      • Beta endothelial cell growth factor antibody
      • Beta-endothelial cell growth factor antibody
      • ECGF antibody
      • ECGF beta antibody
      • ECGF-beta antibody
      • ECGFA antibody
      • ECGFB antibody
      • Endothelial Cell Growth Factor alpha antibody
      • Endothelial Cell Growth Factor beta antibody
      • FGF 1 antibody
      • FGF alpha antibody
      • Fgf1 antibody
      • FGF1_HUMAN antibody
      • FGFA antibody
      • Fibroblast Growth Factor 1 Acidic antibody
      • Fibroblast growth factor 1 antibody
      • GLIO703 antibody
      • HBGF 1 antibody
      • HBGF-1 antibody
      • HBGF1 antibody
      • Heparin binding growth factor 1 antibody
      • Heparin binding growth factor 1 precursor antibody
      • Heparin-binding growth factor 1 antibody
      see all

    画像

    • FGF1 antibody (ab54677) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab54677 FGF1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
    • All lanes : Anti-FGF1 antibody (ab54677) at 1 µg/ml

      Lane 1 : Human kidney whole cell lysate (50µg)
      Lane 2 : Human liver whole cell lysate (50µg)
      Lane 3 : Human spleen whole cell lysate (50µg)
      Lane 4 : Human pancreas whole cell lysate (50µg)
      Lane 5 : Human colon whole cell lysate (50µg)
      Lane 6 : Human placenta whole cell lysate (50µg)
      Lane 7 : Human stomach whole cell lysate (50µg)
      Lane 8 : Mouse brain whole cell lysate (50µg)

      Secondary
      Goat anti-mouse antibody conjugated to HRP at 1/5000 dilution

    参考文献

    ab54677 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Recombinant protein (human)
    Specification
    human
    Blocking step
    Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type
    Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    投稿 Sep 04 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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