製品の概要

  • 製品名Anti-FGF1 antibody [2E12]
    FGF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E12] to FGF1
  • アプリケーション適用あり: WB, IP, ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant Human FGF1 protein.

  • ポジティブ・コントロール
    • Human placenta tissue. HeLa cells. Tranfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab117640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 細胞内局在Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Anti-FGF1 antibody [2E12] 画像

  • Overlay histogram showing HEK293 cells stained with ab117640 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117640, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Human Placenta, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117640 at 5µg/ml.
  • Immunofluorescence analysis of FGF1 expression on HeLa cells with ab117640 at 10µg/ml.
  • All lanes : Anti-FGF1 antibody [2E12] (ab117640)

    Lane 1 : Transfected 293T cell line
    Lane 2 : Non-Transfected 293T cell line


    Predicted band size : 17 kDa
  • The detection limit for ab117640 is approximately 10ng/ml as a capture antibody.

Anti-FGF1 antibody [2E12] (ab117640) 使用論文

ab117640 has not yet been referenced specifically in any publications.

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