製品の概要

  • 製品名Anti-FGF 23 antibody
    FGF 23 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FGF 23
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human FGF 23.

    (Peptide available as ab109073.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human heart; Human bone marrow; Human bone tumour as well as the following whole cell lysates: U2OS; U937; MOLT4.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab98000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

  • 機能Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
  • 組織特異性Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
  • 関連疾患Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
    Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 翻訳後修飾Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
    O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.
  • 細胞内局在Secreted. Secretion is dependent on O-glycosylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADHR antibody
    • FGF-23 antibody
    • Fgf23 antibody
    • FGF23_HUMAN antibody
    • FGFN antibody
    • Fibroblast growth factor 23 antibody
    • Fibroblast growth factor 23 C-terminal peptide antibody
    • Fibroblast growth factor 23 precursor antibody
    • HPDR2 antibody
    • HYPF antibody
    • Phosphatonin antibody
    • PHPTC antibody
    • Tumor derived hypophosphatemia inducing factor antibody
    • Tumor-derived hypophosphatemia-inducing factor antibody
    see all

Anti-FGF 23 antibody 画像

  • All lanes : Anti-FGF 23 antibody (ab98000) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 4 : Human bone tumor tissue lysate - total protein (ab29359)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 48 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-FGF 23 antibody (ab98000) 使用論文

ab98000 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The U2OS cell line is a human osteosarcoma cell line and can be used as positive control. Your WB results look perfectly normal and clean. I am not entirely certain why you feel you cannot trust your results. I am inc...

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