製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Fez1
  • 特異性
    ab53562 is expected to recognize isoform 1 (NP_005094.1) only.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-QNKQKEQRELMKK

    , corresponding to internal sequence amino acids 278-290 of Human Fez1

  • ポジティブ・コントロール
    • Human Brain (frontal Cortex) lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能
    May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
  • 組織特異性
    Mainly expressed in brain.
  • 配列類似性
    Belongs to the zygin family.
  • 翻訳後修飾
    Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.
    Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome. Cell membrane. Co-localizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fasciculation and elongation protein zeta 1 antibody
    • Fasciculation and elongation protein zeta-1 antibody
    • Fez 1 antibody
    • Fez antibody
    • FEZ1 antibody
    • FEZ1_HUMAN antibody
    • Fezl antibody
    • Zygin 1 antibody
    • Zygin I antibody
    • Zygin-1 antibody
    • Zygin1 antibody
    • ZyginI antibody
    see all

画像

  • Anti-Fez1 antibody (ab53562) at 0.03 µg/ml + Human Brain (frontal Cortex) lysate (in RIPA buffer) at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

プロトコール

参考文献

This product has been referenced in:
  • McKnight NC  et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J : (2012). WB ; Human . Read more (PubMed: 22354037) »

See 1 Publication for this product

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