Anti-Ferritin Light Chain 抗体 (ab69090)
Key features and details
- Rabbit polyclonal to Ferritin Light Chain
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Ferritin Light Chain antibody
Ferritin Light Chain 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ferritin Light Chain -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
アプリケーション
適用あり: IHC-FoFr, WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog, Orangutan -
免疫原
Synthetic peptide corresponding to Human Ferritin Light Chain aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab69089) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69090の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr | (1) |
Use a concentration of 1 µg/ml.
|
WB | (21) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa).
|
特記事項 |
---|
IHC-FoFr
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-21 kDa (predicted molecular weight: 19 kDa). |
ターゲット情報
-
機能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
関連疾患
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
配列類似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 477042 Dog
- Entrez Gene: 2512 Human
- Entrez Gene: 14325 Mouse
- Entrez Gene: 100173858 Orangutan
- Entrez Gene: 29292 Rat
- Omim: 134790 Human
- SwissProt: Q53VB8 Dog
- SwissProt: P02792 Human
see all -
別名
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
画像
-
All lanes : Anti-Ferritin Light Chain antibody (ab69090) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 :Mouse spleen tissue lysate - total protein (ab29293)
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lane 5 : Liver (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (78)
ab69090 は 78 報の論文で使用されています。
- Cheng Z et al. Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis. Cancer Sci 113:65-78 (2022). PubMed: 34699654
- Tisdall MD et al. Ex vivo MRI and histopathology detect novel iron-rich cortical inflammation in frontotemporal lobar degeneration with tau versus TDP-43 pathology. Neuroimage Clin 33:102913 (2022). PubMed: 34952351
- Yue L et al. PCBP2 knockdown promotes ferroptosis in malignant mesothelioma. Pathol Int 72:242-251 (2022). PubMed: 35089637
- Raha AA et al. Interplay of Ferritin Accumulation and Ferroportin Loss in Ageing Brain: Implication for Protein Aggregation in Down Syndrome Dementia, Alzheimer's, and Parkinson's Diseases. Int J Mol Sci 23:N/A (2022). PubMed: 35162984
- Michaelis V et al. Differences and Interactions in Placental Manganese and Iron Transfer across an In Vitro Model of Human Villous Trophoblasts. Int J Mol Sci 23:N/A (2022). PubMed: 35328723