製品の概要

  • 製品名Anti-Ferritin Heavy Chain antibody
    Ferritin Heavy Chain 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ferritin Heavy Chain
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Ferritin Heavy Chain aa 108-157 (internal sequence).
    Sequence:

    EKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLR


    Database link: P02794

  • ポジティブ・コントロール
    • Jurket Cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

Anti-Ferritin Heavy Chain antibody 画像



  • Predicted band size : 21 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling Ferritin Heavy Chain with ab81444 at 1.0µg/ml


  • Predicted band size : 21 kDa
    Western blot analysis of HeLa cell lysate labeling Ferritin Heavy Chain with ab81444 at 1.0µg/ml
  • Anti-Ferritin Heavy Chain antibody (ab81444) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Ferritin Heavy Chain with ab81444 at 5 µg/ml.

Anti-Ferritin Heavy Chain antibody (ab81444) 使用論文

This product has been referenced in:
  • Colafrancesco S  et al. sCD163 in AOSD: a biomarker for macrophage activation related to hyperferritinemia. Immunol Res 60:177-83 (2014). Read more (PubMed: 25388964) »
  • Asensio-López MC  et al. Involvement of ferritin heavy chain in the preventive effect of metformin against doxorubicin-induced cardiotoxicity. Free Radic Biol Med : (2012). Read more (PubMed: 23000260) »

See all 2 Publications for this product

Product Wall

We do not have any mature ferritin binding data for the rabbit or chicken polyclonals, or any of the other heavy chain ferritin antibodies. We do, however, have kidney IHC data for the rabbit polyclonal, which suggests it is binding mature ferritin, as...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"