Anti-Ferritin 抗体 [F23] (ab10060)

製品の概要

  • 製品名Anti-Ferritin antibody [F23]
    Ferritin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F23] to Ferritin
  • アプリケーション適用あり: ICC/IF, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Ferritin. Purified human ferritin
    Database link: P02794

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab10060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [F31] to Ferritin (HRP) (ab24475). Can be used as Capture antibody.

ターゲット情報

  • 機能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 配列類似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
  • 別名
    • Cell proliferation-inducing gene 15 protein antibody
    • Ferritin H subunit antibody
    • Ferritin heavy chain antibody
    • Ferritin heavy polypeptide 1 antibody
    • Ferritin L subunit antibody
    • Ferritin light polypeptide antibody
    • Ferritin, heavy polypeptide antibody
    • FRIH_HUMAN antibody
    • FTH antibody
    • FTH1 antibody
    • FTL antibody
    see all

Anti-Ferritin antibody [F23] 画像

  • ICC/IF image of ab10060 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10060, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ferritin antibody [F23] (ab10060) 使用論文

ab10060 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I contacted the source the two antibodies and got the following information:

ab10060 :

This antibody has been tested to work at least with human serum ferritin and liver ferritin.It was not test...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jun 02 2009

Thank you for your enquiry. I have been in touch with the source of our ferritin antibodies and I can tell you that from the feedback that we have received from our customers both monoclonal antibodies; ab10060 and ab24475 are specific to the L-subu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"