Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 93 kDa.
1/50 - 1/100.
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Expressed in a variety of lymphoid cell lines.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
Cytoplasm. Nucleus. Associated with the chromatin.
Information by UniProt
c FER antibody
Feline encephalitis virus related kinase FER antibody
Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
Anti-FER (phospho Y402) antibody 画像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)
ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
Western blot - FER (phospho Y402) antibody (ab79573)
All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution Lane 1 : Jurkat cell extracts, starved (24 hours) Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 93 kDa Observed band size : 93 kDa Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Anti-FER (phospho Y402) antibody (ab79573) 使用論文
This product has been referenced in:
et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"