製品の概要

  • 製品名Anti-FER (phospho Y402) antibody
    FER 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FER (phospho Y402)
  • 特異性ab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E)

  • ポジティブ・コントロール
    • Human brain tissue; Jurkat cell extracts starved for 24 hours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
  • 組織特異性Expressed in a variety of lymphoid cell lines.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
    Contains 1 FCH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 細胞内局在Cytoplasm. Nucleus. Associated with the chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c FER antibody
    • Feline encephalitis virus related kinase FER antibody
    • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
    • Fer (fps/fes related) tyrosine kinase antibody
    • FER antibody
    • FER_HUMAN antibody
    • FerT antibody
    • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
    • p94 FER antibody
    • p94-FER antibody
    • Pe1Fe10 antibody
    • Pe1Fe13 antibody
    • Pe1Fe3 antibody
    • Pe1Fe6 antibody
    • Phosphoprotein NCP94 antibody
    • PPP1R74 antibody
    • Protein phosphatase 1 regulatory subunit 74 antibody
    • Proto oncogene tyrosine protein kinase FER antibody
    • Proto-oncogene c-Fer antibody
    • TYK3 antibody
    • Tyrosine kinase 3 antibody
    • Tyrosine-protein kinase Fer antibody
    see all

Anti-FER (phospho Y402) antibody 画像

  • ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
  • All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, starved (24 hours)
    Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-FER (phospho Y402) antibody (ab79573) 使用論文

This product has been referenced in:
  • Chang SF  et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014). Read more (PubMed: 24449884) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"