Rabbit polyclonal to FER - C-terminal
Synthetic peptide from the C-terminal region of human FER conjugated to KLH.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71682 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Expressed in a variety of lymphoid cell lines.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
Cytoplasm. Nucleus. Associated with the chromatin.
Information by UniProt
c FER antibody
Feline encephalitis virus related kinase FER antibody
Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
Western blot - FER antibody - C-terminal (ab71682)
Anti-FER antibody - C-terminal (ab71682) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size:
Observed band size:
100 kDa (
why is the actual band size different from the predicted?
Additional bands at:
50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
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