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Our Abpromise guarantee covers the use of ab5668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.2 µg/ml. Detects a band of approximately 97 kDa.Can be blocked with FE65 peptide (ab5892).|
|ChIP||Use at an assay dependent concentration. PubMed: 1755654|
Western blot of Fe65-affinity pulldown materials from mouse brain lysate using ab5668. Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.
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