製品の概要

  • 製品名Anti-FCN1 antibody
    FCN1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to FCN1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY, corresponding to amino acids 201-300 of Human FCN1 (NP_001994) with a 26 kDa tag.

  • ポジティブ・コントロール
    • FCN1 transfected 293T cell lysate; Recombinant tagged human FCN1 fragment.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged FCN1 is approximately 0.3ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Involved in serum exerting lectin activity. Binds GlcNAc.
  • 組織特異性Peripheral blood leukocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes and granulocytes.
  • 配列類似性Belongs to the ficolin lectin family.
    Contains 1 collagen-like domain.
    Contains 1 fibrinogen C-terminal domain.
  • 細胞内局在Secreted. Found on the monocyte surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Collagen/fibrinogen domain containing protein 1 antibody
    • Collagen/fibrinogen domain-containing protein 1 antibody
    • Fcn1 antibody
    • FCN1_HUMAN antibody
    • FCNM antibody
    • ficolin (collagen/fibrinogen domain containing) 1 antibody
    • Ficolin 1 antibody
    • Ficolin A antibody
    • Ficolin alpha antibody
    • Ficolin-1 antibody
    • Ficolin-A antibody
    • Ficolin-alpha antibody
    • M ficolin antibody
    • M-ficolin antibody
    see all

Anti-FCN1 antibody 画像

  • Anti-FCN1 antibody (ab77454) at 1 µg/ml + Recombinant tagged human FCN1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FCN1 antibody (ab77454) at 1 µg/ml

    Lane 1 : FCN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-FCN1 antibody (ab77454) 使用論文

ab77454 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77454.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"