Anti-FCGR1A 抗体 (ab104273)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FCGR1A
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Dog, Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within internal amino acids 225-255 from Human FCGR1A (P12314)

  • ポジティブ・コントロール
    • NCI-H460 cell lysate, Human brain tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab104273 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
  • 組織特異性
    Monocyte/macrophage specific.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. FCGR1 family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾
    Phosphorylated on serine residues.
  • 細胞内局在
    Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD64 antibody
    • CD64A antibody
    • Fc fragment of IgG high affinity Ia receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc-gamma RI antibody
    • Fc-gamma RIA antibody
    • FCG1 antibody
    • FcgammaRIa antibody
    • FCGR1 antibody
    • FCGR1_HUMAN antibody
    • FCGR1A antibody
    • FcRI antibody
    • FLJ18345 antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • IGFR1 antibody
    • IgG Fc receptor I antibody
    • Immunoglobulin G Fc receptor I antibody
    • OTTHUMP00000013913 antibody
    see all

画像

  • Anti-FCGR1A antibody (ab104273) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size : 43 kDa
  • ab104273, at a 1/50 dilution, staining FCGR1A in formalin fixed and paraffin embedded Human brain tissue, followed by peroxidase conjugation of the secondary antibody and DAB staining.

参考文献

ab104273 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0 in steamer for 30 minutes
Sample
Dog Tissue sections (Brain tissue (microglia, astrocytes) and leukocyte)
Specification
Brain tissue (microglia, astrocytes) and leukocyte
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Guilherme Dias de Melo

Verified customer

投稿 Jul 17 2013

Thank you for that feedback. I am pleased to hear that the replacement antibody has been working more successfully than the original anti-CD64 antibody.


I, like you, havenot been able tofind much referenceto CD64 beingexpressed in epith...

Read More

I have issued the free of charge antibody ab104273, which should be with you on Monday. The order number is xxxxxx. I hope the suggestions that I have made if not the new antibody will help. I wish you well with your experiments. If you do cont...

Read More

Thank you for providing that extra information, and sorry for the delay in getting back to you. I wanted to make sure that I could help you in the best way possible before responding. I have been in contact with the source of this antibody (ab11984...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録