製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FCER1G
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT LLYCRLKIQV) of Human FCER1G (NP_004097).

  • ポジティブ・コントロール
    • HeLa cell lysate IHC-P (FFPE): Human Lung (Normal)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Associates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface.
  • 配列類似性
    Belongs to the CD3Z/FCER1G family.
    Contains 1 ITAM domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide antibody
    • Fc receptor gamma chain antibody
    • Fc-epsilon RI-gamma antibody
    • FCER1G antibody
    • FCERG_HUMAN antibody
    • FceRI gamma antibody
    • FCRG antibody
    • FcRgamma antibody
    • High affinity immunoglobulin epsilon receptor subunit gamma antibody
    • IgE Fc receptor subunit gamma antibody
    • Immunoglobulin E receptor, high affinity, gamma chain antibody
    see all

画像

  • IHC image of FCER1G staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108228, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-FCER1G antibody (ab108228) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 10 kDa
    10-20% gel

参考文献

ab108228 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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