Anti-Fc epsilon RI 抗体 [0.T.195] (ab33568)

製品の概要

  • 製品名Anti-Fc epsilon RI antibody [0.T.195]
    Fc epsilon RI 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [0.T.195] to Fc epsilon RI
  • 特異性This antibody recognizes Fc epsilon RI.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Whole rat basophilic leukemia cells, positive for the rat high affinity receptor recognizing immunoglobulin E (Fc epsilon RI).

  • ポジティブ・コントロール
    • Biotinylated rat basophilic leukemia cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名0.T.195
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. 5 µg immunoprecipitates Fc epsilon RI from 500 µg of cell lysate.

ターゲット情報

  • 機能Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • 配列類似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • Fcer1a antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

Anti-Fc epsilon RI antibody [0.T.195] 画像

  • ab33568 staining Fc epsilon RI (red) in Rat mast cells (RBL) by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.15 Triton X-100 prior to blocking in 1% BSA and 1% FBS for 30 minutes at 22°C. The primary antibody was diluted 1/500 in PBS with 1% BSA and 1% FBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 647-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

    See Abreview

Anti-Fc epsilon RI antibody [0.T.195] (ab33568) 使用論文

ab33568 has not yet been referenced specifically in any publications.

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Thank you for your email.
We recommend storing the antibody at -20C after immediately after receiving it or after 1-2 weeks of ordering. We do not provide guarantee if the antibodies aren't stored as recommended. In your case the antibody was store...

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Thank you for contacting us. I am sorry to hear about the problem you are experiencing.
I have few question to ask before making any further decision.
- Have you spin the vial in centrifuge?
- Did you open the vial after receiving it and th...

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I am very pleased to hear you would like to accept our offer and test ab33568 in blocking. This code will give you 1 free primary antiody before the expiration date. To redeem this offer, please submit an Abreview for blocking and include this code in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mast cell - RBL)
Specification Mast cell - RBL
Fixative Paraformaldehyde
Permeabilization Yes - I have samples with and without permeabilization- 0.1%triton
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Nurit Pereg-Azouz

Verified customer

投稿 Mar 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"