The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Use at an assay dependent concentration. PubMed: 24936062
Is unsuitable for IP.
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
Protein modification; protein ubiquitination.
Belongs to the FBXO31 family. Contains 1 F-box domain.
Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
Abdullah A et al. Murine Myocardial Transcriptome Analysis Reveals a Critical Role of COPS8 in the Gene Expression of Cullin-RING Ligase Substrate Receptors and Redox and Vesicle Trafficking Pathways. Front Physiol8:594 (2017).
Read more (PubMed: 28861005) »
Liu J et al. F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6). J Biol Chem289:21508-18 (2014).
Read more (PubMed: 24936062) »