The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90,148 kDa (predicted molecular weight: 132 kDa).
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 3 of FBXL11. The predicted molecular weights of isoforms 1 and 3 are 132 kDa and 90 kDa respectively. FBXL11 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Use a concentration of 1 µg/ml.
1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use at an assay dependent concentration. PubMed: 23386205
Use at an assay dependent concentration. PubMed: 21029866
機能Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
組織特異性Widely expressed, with highest levels in brain, testis and ovary, followed by lung.
ドメインThe JmjC domain mediates demethylation activity and is required for satellite silencing. The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab31739 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ICC/IF image of ab31739 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31739, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas ab31739 staining KDM2A in human skin, showing a distinct nuclear staining pattern in the epidermis. Paraffin-embedded tissue sections (4µm) were incubated with ab31739 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval by heat induction in citrate buffer pH 6. ab31739 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Anti-FBXL11 antibody - ChIP Grade (ab31739) 使用論文
This product has been referenced in:
Dong R et al. Depletion of histone demethylase KDM2A enhanced the adipogenic and chondrogenic differentiation potentials of stem cells from apical papilla. Exp Cell ResN/A:N/A (2013).
Read more (PubMed: 23872478) »
Bartke T et al. Nucleosome-interacting proteins regulated by DNA and histone methylation. Cell143:470-84 (2010).
Read more (PubMed: 21029866) »