Anti-KDM2A 抗体 (ab99242)
Key features and details
- Rabbit polyclonal to KDM2A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-KDM2A antibody
KDM2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KDM2A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat -
免疫原
Synthetic peptide corresponding to Human KDM2A aa 800-900.
Database link: NP_036440.1 -
ポジティブ・コントロール
- Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Skin Basal Cell Carcinoma and Stomach Adenocarcinoma
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99242の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
|
特記事項 |
---|
IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
ターゲット情報
-
機能
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. -
組織特異性
Widely expressed, with highest levels in brain, testis and ovary, followed by lung. -
配列類似性
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
ドメイン
The JmjC domain mediates demethylation activity and is required for satellite silencing.
The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. - Information by UniProt
-
参照データベース
- Entrez Gene: 483702 Dog
- Entrez Gene: 100052955 Horse
- Entrez Gene: 22992 Human
- Entrez Gene: 100294703 Pig
- Omim: 605657 Human
- SwissProt: Q9Y2K7 Human
- Unigene: 124147 Human
-
別名
- [Histone-H3]-lysine-36 demethylase 1A antibody
- CXXC-type zinc finger protein 8 antibody
- CXXC8 antibody
see all
画像
-
ab99242, at 1/250 dilution, staining KDM2A in formalin-fixed paraffin-embedded Human skin basal cell carcinoma by Immunohistochemistry using DAB staining.
-
ab99242, at 1/250 dilution, staining KDM2A in formalin-fixed paraffin-embedded Human breast carcinoma by Immunohistochemistry using DAB staining.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab99242 は 2 報の論文で使用されています。
- Okamoto K et al. KDM2A-dependent reduction of rRNA transcription on glucose starvation requires HP1 in cells, including triple-negative breast cancer cells. Oncotarget 10:4743-4760 (2019). PubMed: 31413816
- Okamoto K et al. SF-KDM2A binds to ribosomal RNA gene promoter, reduces H4K20me3 level, and elevates ribosomal RNA transcription in breast cancer cells. Int J Oncol N/A:N/A (2017). PubMed: 28350064