Anti-Fbx32 抗体 (ab92281)
Key features and details
- Goat polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fbx32 antibody
Fbx32 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Fbx32 -
由来種
Goat -
特異性
Expected to recognize reported isoform 1 (NP_478136.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Cow, Human, Pig -
免疫原
Synthetic peptide:
C-NSKTKTQYFHQEK
, corresponding to internal amino acids 70-82 of Human Fbx32 (NP_478136.1).(Peptide available as ab131394.) -
ポジティブ・コントロール
- WB: Mouse skeletal muscle lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92281の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
組織特異性
Specifically expressed in cardiac and skeletal muscle. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 F-box domain. - Information by UniProt
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参照データベース
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
see all -
別名
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (10)
ab92281 は 10 報の論文で使用されています。
- Järve A et al. Angiotensin-(1-7) Receptor Mas Deficiency Does Not Exacerbate Cardiac Atrophy Following High-Level Spinal Cord Injury in Mice. Front Physiol 11:203 (2020). PubMed: 32226394
- Mose M et al. A model mimicking catabolic inflammatory disease; a controlled randomized study in humans. PLoS One 15:e0241274 (2020). PubMed: 33151986
- Møller AB et al. Molecular and cellular adaptations to exercise training in skeletal muscle from cancer patients treated with chemotherapy. J Cancer Res Clin Oncol 145:1449-1460 (2019). PubMed: 30968255
- Dalgaard LB et al. Influence of Oral Contraceptive Use on Adaptations to Resistance Training. Front Physiol 10:824 (2019). PubMed: 31312144
- Møller AB et al. Immobilization Decreases FOXO3a Phosphorylation and Increases Autophagy-Related Gene and Protein Expression in Human Skeletal Muscle. Front Physiol 10:736 (2019). PubMed: 31258486
- Majumder A et al. Hydrogen sulfide alleviates hyperhomocysteinemia-mediated skeletal muscle atrophy via mitigation of oxidative and endoplasmic reticulum stress injury. Am J Physiol Cell Physiol 315:C609-C622 (2018). PubMed: 30110564
- Wilkinson DJ et al. Impact of the calcium form of ß-hydroxy-ß-methylbutyrate upon human skeletal muscle protein metabolism. Clin Nutr N/A:N/A (2017). PubMed: 29097038
- Apró W et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
- Vendelbo MH et al. Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling. PLoS One 9:e102031 (2014). WB ; Human . PubMed: 25020061
- Borgenvik M et al. Intake of branched-chain amino acids influences the levels of MAFbx mRNA and MuRF-1 total protein in resting and exercising human muscle. Am J Physiol Endocrinol Metab 302:E510-21 (2012). WB ; Human . PubMed: 22127230