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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Fbx32.
(Peptide available as ab74022.)
Our Abpromise guarantee covers the use of ab74023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab74023 (1/2000) staining Fbx32 in paraffin-embedded Human heart tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.
ab74023 staining Fbx32 in rat muscle tissue sections by Immunohistochemistry (Frozen section). Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 at a 1/200 dilution, for 24 hours at 4°C. A pig anti-rabbit Alexa Fluoro 488 conjugate was used as secondary antibody at a 1/1000 dilution. Nuclei were stained using the standard Hoestch method and are depicted in blue.
ab74023 staining cultured rat astrocytes by ICC/IF. The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured astrocytes were then stained with ab74023 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. FBX32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.