製品の概要

  • 製品名Anti-FBP1 antibody
    FBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FBP1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1 - 338 of Human FBP1 (NP_000498.2).

  • ポジティブ・コントロール
    • Mouse liver tissue lysate and transfected 293T cell lysate IF/ICC: HeLa cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
  • 配列類似性Belongs to the FBPase class 1 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6-bisphosphatase 1 antibody
    • 6-bisphosphate 1-phosphohydrolase 1 antibody
    • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
    • D-fructose-1 antibody
    • EC 3.1.3.11 antibody
    • F16P1_HUMAN antibody
    • FBP antibody
    • FBP 1 antibody
    • FBP1 antibody
    • FBPase 1 antibody
    • Fructose 1 6 bisphosphatase 1 antibody
    • Fructose bisphosphatase 1 antibody
    • Fructose-1 antibody
    • Growth inhibiting protein 17 antibody
    • Liver fructose bisphosphatase antibody
    see all

Anti-FBP1 antibody 画像

  • ICC/IF image of ab103165 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103165, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FBP1 antibody (ab103165) at 1 µg/ml + mouse liver tissue lysate at 50 µg

    Predicted band size : 37 kDa
  • All lanes : Anti-FBP1 antibody (ab103165) at 1 µg/ml

    Lane 1 : FBP1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa

Anti-FBP1 antibody (ab103165) 使用論文

ab103165 has not yet been referenced specifically in any publications.

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