Anti-Fatty Acid Synthase 抗体 (ab22759)
Key features and details
- Rabbit polyclonal to Fatty Acid Synthase
- Suitable for: WB, IHC (PFA fixed), ICC
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Fatty Acid Synthase antibody
Fatty Acid Synthase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fatty Acid Synthase -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab128870.
-
アプリケーション
適用あり: WB, IHC (PFA fixed), ICCmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow -
免疫原
Synthetic peptide corresponding to Mouse Fatty Acid Synthase aa 2450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab25719) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22759の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (7) |
Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
|
IHC (PFA fixed) |
Use a concentration of 2 µg/ml.
|
|
ICC |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa). |
IHC (PFA fixed)
Use a concentration of 2 µg/ml. |
ICC
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
組織特異性
Ubiquitous. Prominent expression in brain, lung, and liver. -
配列類似性
Contains 1 acyl carrier domain. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
参照データベース
- Entrez Gene: 281152 Cow
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P12276 Chicken
- SwissProt: Q71SP7 Cow
- SwissProt: P49327 Human
see all -
別名
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
画像
-
ab22759 staining Fatty Acid Synthase in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22759 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Hu liver tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab22759 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab22759 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab22759 and ab18058 (loading control to Vinculin) were diluted at 1 µg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Fatty Acid Synthase antibody (ab22759) at 1 µg/ml
Lane 1 : 3T3-L1 nuclear extract lysate (ab14632)
Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
Lane 4 : 3T3-L1 nuclear extract lysate (ab14632) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
Lane 5 : Brain (Mouse) Tissue Lysate (ab27253) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
Lane 6 : Liver (Mouse) Tissue Lysate (ab7935) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 273 kDa
Observed band size: 273 kDa
Additional bands at: 100 kDa, 35 kDa, 50 kDa (possible IgG). We are unsure as to the identity of these extra bands. -
Immunofluorescent staining for Fatty Acid Synthase in the rat striatum using Rabbit polyclonal to Fatty Acid Synthase (ab22759). Abundant staining was observed in the Striatum with lower levels of staining observed in the Corpus callosum. This is a montage of three pictures aquired using a X10 objective. ab22759 was used at 1/200 (2µg/ml) incubated overnight at room temperature.
Secondary antibody used was anti-rabbit Alexa Fluor® 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (100)
ab22759 は 100 報の論文で使用されています。
- Yu PW et al. EGCG Restricts PRRSV Proliferation by Disturbing Lipid Metabolism. Microbiol Spectr 10:e0227621 (2022). PubMed: 35404086
- Sultan F et al. Temporal analysis of melanogenesis identifies fatty acid metabolism as key skin pigment regulator. PLoS Biol 20:e3001634 (2022). PubMed: 35584084
- Gustafson KT et al. cyc-DEP: Cyclic immunofluorescence profiling of particles collected using dielectrophoresis. Electrophoresis 43:1784-1798 (2022). PubMed: 35753078
- Li Y et al. Redox-Unlockable Nanoparticle-Based MST1 Delivery System to Attenuate Hepatic Steatosis via the AMPK/SREBP-1c Signaling Axis. ACS Appl Mater Interfaces 14:34328-34341 (2022). PubMed: 35858286
- Zhang Q et al. Bailing capsule (Cordyceps sinensis) ameliorates renal triglyceride accumulation through the PPARα pathway in diabetic rats. Front Pharmacol 13:915592 (2022). PubMed: 36091833