製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FASTK
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Pig
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 396-521 of Human FASTK (NP_006703)

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or NIH3T3 cell lysates ICC/IF: HeLa cells

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

画像

  • All lanes : Anti-FASTK antibody (ab97544) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa

  • Anti-FASTK antibody (ab97544) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 61 kDa

  • Anti-FASTK antibody (ab97544) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg

    Predicted band size: 61 kDa

  • Immunohistochemical staining of paraffin-embedded SCC15 xenograft, using ab97544 at 1/100 dilution.

  • Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.

  • FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).

  • All lanes : Anti-FASTK antibody (ab97544) at 1/500 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
    Lower image is co-stained with Hoechst 33342.

参考文献

This product has been referenced in:
  • Zhi F  et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One 8:e72390 (2013). WB . Read more (PubMed: 24013584) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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