Anti-fast skeletal Myosin 抗体 [Y32], prediluted (ab909)

製品の概要

  • 製品名Anti-fast skeletal Myosin antibody [Y32], prediluted
    fast skeletal Myosin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Y32] to fast skeletal Myosin, prediluted
  • 特異性This antibody recognizes heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule.
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Human
  • 免疫原

    Full length protein (Rabbit).

  • ポジティブ・コントロール
    • IHC-P: FFPE human skeletal msucle tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • ポリ/モノモノクローナル
  • クローン名Y32
  • ミエローマunknown
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
  • 追加情報This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
  • ターゲット情報

    Anti-fast skeletal Myosin antibody [Y32], prediluted 画像

    • IHC image of fast skeletal Myosin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab909, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

    Anti-fast skeletal Myosin antibody [Y32], prediluted (ab909) 使用論文

    This product has been referenced in:
    • Haider KH  et al. MicroRNA-21 is a key determinant in IL-11/Stat3 anti-apoptotic signalling pathway in preconditioning of skeletal myoblasts. Cardiovasc Res 88:168-78 (2010). IHC-Fr ; Rat . Read more (PubMed: 20498256) »
    • Barker T  et al. Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery. Free Radic Biol Med 47:1611-8 (2009). IHC-P ; Human . Read more (PubMed: 19751822) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab909.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"