Mouse monoclonal [MYSN02] to Fast Myosin Skeletal Heavy chain, prediluted
ab75370 may be used for detecting cross striated muscle differentiation in tumors. It will not stain human or animal cardiac muscle, smooth muscle myosin, or cells grown by tissue culture (non muscle myosin).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Ready to use.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Contains 1 IQ domain. Contains 1 myosin head-like domain.
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cytoplasm > myofibril. Thick filaments of the myofibrils.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fast Myosin Skeletal Heavy chain antibody [MYSN02 ], prediluted (ab75370)
ab75370 staining Fast Myosin Skeletal Heavy chain in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry, using peroxidase conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.
Lundgreen K et al. Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears. Acta Orthop84:565-70 (2013).
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