Anti-Fast Myosin Skeletal Heavy chain 抗体 (ab91506)

製品の概要

  • 製品名Anti-Fast Myosin Skeletal Heavy chain antibody
    Fast Myosin Skeletal Heavy chain 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fast Myosin Skeletal Heavy chain
  • アプリケーション適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Guinea pig, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fast Myosin Skeletal Heavy chain.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Skeletal Muscle (Human) Tissue Lysate; Skeletal Muscle (Mouse) Tissue Lysate; Skeletal Muscle (Rat) Tissue Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 23515448
WB Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

Anti-Fast Myosin Skeletal Heavy chain antibody 画像

  • All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa


    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) 使用論文

This product has been referenced in:
  • Ackermann KA  et al. Early changes of muscle membrane properties in porcine faecal peritonitis. Crit Care 18:484 (2014). WB ; Pig . Read more (PubMed: 25145497) »
  • Stuart CA  et al. Slow-twitch fiber proportion in skeletal muscle correlates with insulin responsiveness. J Clin Endocrinol Metab 98:2027-36 (2013). IHC-Fr ; Human . Read more (PubMed: 23515448) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Rat Cell (Muscle Tissue)
Permeabilization Yes - TritonX 0,1%
Specification Muscle Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Acetone
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投稿 Nov 02 2016

Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Methanol
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投稿 Oct 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"