製品の概要

  • 製品名Anti-Fas Ligand antibody [SB93a] (FITC)
    Fas Ligand 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SB93a] to Fas Ligand (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性Reacts with native and recombinant Human Fas Ligand.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant Human protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µl for 106 cells. ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • 関連疾患Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • 細胞内局在Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

Anti-Fas Ligand antibody [SB93a] (FITC) 画像

  • Flow Cytometry analysis of 1,10-Phenanthroline-stimulated human peripheral blood lymphocytes labeling Fas Ligand with ab87023 at 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG2b-FITC.

  • ab87023 at 1µg/ml, staining Human PBMCs stimulated with (red line) PMA/ionomycin, then blocked with an MMP7 inhibitor (dotted line corresponds to unstimulated cells)

Anti-Fas Ligand antibody [SB93a] (FITC) (ab87023) 使用論文

This product has been referenced in:
  • Bhogal RH  et al. Activation of CD40 with Platelet Derived CD154 Promotes Reactive Oxygen Species Dependent Death of Human Hepatocytes during Hypoxia and Reoxygenation. PLoS One 7:e30867 (2012). Flow Cyt ; Human . Read more (PubMed: 22295117) »
  • Carney EF  et al. Classical ataxia telangiectasia patients have a congenitally aged immune system with high expression of CD95. J Immunol 189:261-8 (2012). Human . Read more (PubMed: 22649200) »

See all 2 Publications for this product

Product Wall


For stimulation, 1 µg/ml of PMA (Sigma, P1585) and 5 µg/ml of Ionomycin (Sigma, I0634) were added to cells and incubated for 2 hours. For the last 30 minutes of this 2 hour incubation, 0.1mM of 10PM (I, 10- Phenanthroline Monohydrate) ...

Read More

The final concentration of 10PM should be around 0.1mM. The 1µg/mL of PMA and 5µg/mL of Ionomycin are the final concentrations in the cell supsension. Also, when adding 10PM, add it in the last thirty minutes of the 2 hour stimulation.

While we believe that this product will work with soluble Fas Ligand, we have not tested this antibody to confirm.

The protocol for the positive control for flow cytometry is given below:

1 µg/ml of PMA (Sigma, P1585) and 5 &mic...

Read More

Thank you for your email. Please find below the answers to your questions : 1. I would like to know how many cells you used for the stimulation, Answer : Typically we use approximately 0.5 x 106 cells per tube 2. the concentration of PM...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"