製品の概要

  • 製品名Anti-Fas Ligand antibody
    Fas Ligand 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fas Ligand
  • アプリケーション適用あり: WB, IHC-P, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Fas Ligand (C terminal). Different to the related mouse sequence by three amino acids.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF-7.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml.
ICC Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • 関連疾患Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • 細胞内局在Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

Anti-Fas Ligand antibody 画像

  • Anti-Fas Ligand antibody (ab68338) at 1 µg/ml + HeLa cell lysate

    Predicted band size : 31 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab68338 staining Fas Ligan in human intestinal cancer tissue by Immunohistochemistry (formalin fixed, paraffin embedded section). Primary antibody used at 2µg/ml.
  • Anti-Fas Ligand antibody (ab68338) at 1/500 dilution + Mouse serum at 50 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa (possible isoform).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab68338 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68338 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Fas Ligand antibody (ab68338) 使用論文

This product has been referenced in:
  • Hong CS  et al. Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer. J Extracell Vesicles 5:29289 (2016). WB ; Human . Read more (PubMed: 27018366) »
  • Yeh CN  et al. Fas/Fas Ligand Mediates Keratinocyte Death in Sunitinib-Induced Hand-Foot Skin Reaction. J Invest Dermatol N/A:N/A (2014). Read more (PubMed: 24802849) »

See all 6 Publications for this product

Product Wall

Thank you very much for your call today and for your questions about ab68338.

We do havea positive Abreview showing the antibody working in a Western blot with mouse serum-

http://www.abcam.com/index.html?datasheet=68338&tab=abr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Serum (Mouse serum)
Loading amount 50 µg
Specification Mouse serum
Gel Running Conditions Reduced Denaturing (4-20% pre-cast gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Aug 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"