製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Fas Ligand
  • アプリケーション
    適用あり: IHC-Fr, ICC, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Fas Ligand aa 1-60 (N terminal). The exact sequence is proprietary. Antibody will not interact with the soluble FasL corresponding to the C-term of the protein, the extracellular domain is in aa103-281. It will only react with the membrane form.

  • ポジティブ・コントロール
    • Prostate tissue slides.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • 関連疾患
    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性
    Belongs to the tumor necrosis factor family.
  • 翻訳後修飾
    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • 細胞内局在
    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

画像

  • ab15285 staining Fas Ligand in mouse skeletal muscle blood vessel tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/100 in PBS-Tween 20) at 4°C for 12 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody.

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  • ab15285 at 1/100 dilution staining Fas Ligand in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab15285 staining Fas Ligand in Mouse macrophage RAAW264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/1000).

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  • ab15285 staining Fas Ligand in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

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  • ab15285 staining Fas Ligand in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

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参考文献

This product has been referenced in:
  • Liu G  et al. Beclin-1-mediated Autophagy Protects Against Cadmium-activated Apoptosis via the Fas/FasL Pathway in Primary Rat Proximal Tubular Cell Culture. Sci Rep 7:977 (2017). Read more (PubMed: 28428545) »
  • Xiao J  et al. Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Invest Ophthalmol Vis Sci 58:1801-1810 (2017). Read more (PubMed: 28346613) »

See all 32 Publications for this product

レビューと Q&A

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Bone marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6,0
Permeabilization
No
Specification
Bone marrow
Blocking step
BSA+MILK+BFS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 18 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 17 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Tissue lysate - whole (uterus)
Specification
uterus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

投稿 Nov 14 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Tissue sections (Placenta)
Specification
Placenta
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

投稿 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat insulinoma cells)
Loading amount
20 µg
Specification
rat insulinoma cells
Gel Running Conditions
Reduced Denaturing (SDS boiling for 5 min)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Nov 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human Retina)
Specification
Human Retina
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2a)
Loading amount
6 µg
Specification
Neuro2a
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Aug 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (B50 cell line)
Loading amount
6 µg
Specification
B50 cell line
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (33B)
Specification
33B
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jul 06 2012

1-10 of 22 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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