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Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH
Our Abpromise guarantee covers the use of ab82419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 16339059|
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum (ab7475) for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal (ab150073), used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
ab82419 staining CD95 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) ab150073) was used as the secondary antibody.