製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Fas
  • 特異性
    Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Alligator
  • 免疫原

    Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH

  • ポジティブ・コントロール
    • Rat and Mouse brain tissue extracs; Human hepatoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 16339059
ICC/IF 1/50.
IHC-P 1/50.
IHC-Fr 1/50.
ICC 1/50.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • 組織特異性
    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • 関連疾患
    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • 配列類似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • ドメイン
    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

画像

  • All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution

    Lane 1 : Rat brain tissue extract
    Lane 2 : Mouse brain tissue extract

    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum (ab7475) for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal (ab150073), used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    See Abreview

  • ab82419 staining CD95 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) ab150073) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Liu G  et al. Beclin-1-mediated Autophagy Protects Against Cadmium-activated Apoptosis via the Fas/FasL Pathway in Primary Rat Proximal Tubular Cell Culture. Sci Rep 7:977 (2017). Read more (PubMed: 28428545) »
  • Yang Y  et al. Silencing of karyopherin a2 inhibits cell growth and survival in human hepatocellular carcinoma. Oncotarget 8:36289-36304 (2017). WB . Read more (PubMed: 28422734) »

See all 19 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Specification
Brain sections
Blocking step
BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
Fixative
Formaldehyde
Username

Dr. Marcelo Pelajo Machado

Verified customer

投稿 Jul 21 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 17 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Retina)
Specification
Retina
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 17 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

投稿 Nov 12 2013

Thank you for your enquiry and your interest in our products.

I can confirm that ab82419 has been tested for these applications i.e. Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ICC, WB.

However, we have not characterized it for induction/funct...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (RAW267.4 cell line)
Specification
RAW267.4 cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (33B Cell line)
Specification
33B Cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (B50 Neuroblastoma cell line)
Specification
B50 Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophage RAW264.7 cell line)
Specification
Macrophage RAW264.7 cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 25 2012

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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