製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FANCM
  • アプリケーション
    適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    A region within N terminal amino acids 1-50 (MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL) of Human FANCM (NP_065988.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab95014 was affinity purified using an epitope specific to FANCM immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB 1/2000 - 1/10000. Predicted molecular weight: 227 kDa.
IP Use at 5-15 µg/mg of lysate.

ターゲット情報

画像

  • ab95014 staining FANCM in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 2% Triton X-100 prior to blocking in 7% serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 0.1% Tween 20 in PBS and incubated with the sample for 17 hours at 4°C. The secondary antibody was Alexa Fluor® 555 conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.

    See Abreview

  • All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 227 kDa


    Exposure time : 10 seconds
  • ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP.
    Lane 1: ab95014 at 10 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds

参考文献

This product has been referenced in:
  • Jamsai D  et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis 5:e979061 (0). Read more (PubMed: 26413409) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 2% TritonX10
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
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投稿 Mar 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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