The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
機能Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
パスウェイProtein modification; protein ubiquitination.
関連疾患Fanconi anemia complementation group L
配列類似性Contains 1 RING-type zinc finger.
ドメインThe UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
翻訳後修飾The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.