製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FANCI
  • アプリケーション
    適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human FANCI aa 200-250. The exact sequence is proprietary.
    Database link: Q9NVI1

  • ポジティブ・コントロール
    • WB: HeLa cell nuclear extract. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19465922
IP Use at 1-4 µg/mg of lysate.
WB 1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).

ターゲット情報

  • 機能
    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • 関連疾患
    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • ドメイン
    The C-terminal 30 residues are probably required for function in DNA repair.
  • 翻訳後修飾
    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • 細胞内局在
    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FANCI antibody
    • FANCI gene antibody
    • FANCI_HUMAN antibody
    • Fanconi anemia group I protein antibody
    • Fanconi anemia, complementation group I antibody
    • FLJ10719 antibody
    • FLJ14658 antibody
    • KIAA1794 antibody
    • Protein FACI antibody
    • Protein FANCI antibody
    see all

画像

  • ab15344 (1/400) staining FANCI in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

  • All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution

    Lane 1 : HeLa nuclear extract at 5 µg
    Lane 2 : HeLa nuclear extract at 20 µg

    Developed using the ECL technique

    Predicted band size : 149, 142, 27 kDa


    Exposure time : 15 minutes

参考文献

This product has been referenced in:
  • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). Read more (PubMed: 28575672) »
  • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . Read more (PubMed: 23911284) »

See all 7 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 31 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 25 2014

Thank you for submitting an Abreview of ab15344. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

Read More
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cell line)
Total protein in input
40 µg
Specification
HeLa cell line
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Thank you for your interest in our antibodies. We do not provide samples of our antibodies as under our Abpromise we guarantee our antibodies to work as stated on the data sheet. In the case of ab15344, ab74332 and ab15346as they are only guaranteed to...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Treatment
0.5µg/mL MMC for 24h
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録