製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to FANCG
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Pig, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    LEEFRTSLPKSCDL

    , corresponding to C terminal amino acids 609-622 of Human FANCG

  • ポジティブ・コントロール
    • HeLa cell lysate, Jurkat cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
    • 組織特異性
      Highly expressed in testis and thymus. Found in lymphoblasts.
    • 関連疾患
      Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
    • 配列類似性
      Contains 4 TPR repeats.
    • 細胞内局在
      Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DNA repair protein XRCC9 antibody
      • FAG antibody
      • FANCG antibody
      • FANCG_HUMAN antibody
      • Fanconi anaemia complementation group G antibody
      • Fanconi anemia group G protein antibody
      • Protein FACG antibody
      • X ray repair, complementing defective, in Chinese hamster cells 9 antibody
      • X-ray repair, complementing defective, in Chinese hamster, 9 antibody
      • XRCC9 antibody
      see all

    画像

    • Anti-FANCG antibody (ab77193) at 0.5 µg/ml + Jurkat nuclear lysate in RIPA buffer at 35 µg

      Predicted band size : 69 kDa
    • Anti-FANCG antibody (ab77193) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 69 kDa
      Observed band size : 69 kDa


      Exposure time : 1 hour

    プロトコール

    参考文献

    ab77193 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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