Anti-FANCD2 (phospho S1404) 抗体 [EPR2278(2)] (ab109542)


  • 製品名Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)]
    FANCD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2278(2)] to FANCD2 (phospho S1404)
  • 特異性ab109542 only detects FANCD2 phosphorylated at Serine 1404.
  • アプリケーション適用あり: ICC/IF, WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 1404 of Human FANCD2.

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab109542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200. Use with paraformaldehyde fixed cells, permeabilized with 0.5% Triton X-100.
WB 1/1000 - 1/10000. Predicted molecular weight: 166 kDa.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
    • 組織特異性Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
    • 関連疾患Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • 発生段階Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
    • ドメインThe C-terminal 24 residues of isoform 2 are required for its function.
    • 翻訳後修飾Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
      Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
    • 細胞内局在Nucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp762A223 antibody
      • FA 4 antibody
      • FA D2 antibody
      • FA4 antibody
      • FAC D2 antibody
      • FACD 2 antibody
      • FACD antibody
      • FACD2 antibody
      • FACD2_HUMAN antibody
      • FAD antibody
      • FAD2 antibody
      • FANC D2 antibody
      • FANCD 2 antibody
      • FANCD antibody
      • FANCD2 antibody
      • Fanconi anemia complementation group D2 antibody
      • Fanconi anemia group D2 protein antibody
      • FLJ23826 antibody
      • OTTHUMP00000158853 antibody
      • OTTHUMP00000207925 antibody
      • Protein FACD2 antibody
      • Type 4 Fanconi pancytopenia antibody
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    Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] 画像

    • All lanes : Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (ab109542) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase.

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 166 kDa
    • ab109542 (1/200) staining FANCD2 (phospho S1404) in HeLa cells (green). Cells were treated with Bleomycin for 2hrs to induce DNA damage, fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (ab109542) 使用論文

    ab109542 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Nov 15 2011