製品の概要

  • 製品名Anti-FANCD2 antibody [103]
    FANCD2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [103] to FANCD2
  • アプリケーション適用あり: ICC/IF, ELISA, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human FANCD2 fusion protein

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Affinity purified from mouse ascitic fluid.
  • ポリ/モノモノクローナル
  • クローン名103
  • ミエローマNS1
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 17643815
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 155, 170 kDa (predicted molecular weight: 176.5 kDa).
Flow Cyt Use 1µg for 106 cells.

ターゲット情報

  • 機能Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
  • 組織特異性Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
  • 関連疾患Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 発生段階Highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
  • ドメインThe C-terminal 24 residues of isoform 2 are required for its function.
  • 翻訳後修飾Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
    Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
  • 細胞内局在Nucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp762A223 antibody
    • FA 4 antibody
    • FA D2 antibody
    • FA4 antibody
    • FAC D2 antibody
    • FACD 2 antibody
    • FACD antibody
    • FACD2 antibody
    • FACD2_HUMAN antibody
    • FAD antibody
    • FAD2 antibody
    • FANC D2 antibody
    • FANCD 2 antibody
    • FANCD antibody
    • FANCD2 antibody
    • FANCONI ANEMIA COMPLEMENTATION GROUP D antibody
    • Fanconi anemia complementation group D2 antibody
    • Fanconi anemia group D2 protein antibody
    • FANCONI PANCYTOPENIA TYPE 4 antibody
    • FLJ23826 antibody
    • OTTHUMP00000158853 antibody
    • OTTHUMP00000207925 antibody
    • Protein FACD2 antibody
    • Type 4 Fanconi pancytopenia antibody
    see all

Anti-FANCD2 antibody [103] 画像



  • Predicted band size : 176.5 kDa


    ab12450 staining human FANCD2 in HeLa whole cell lysate by Western blot (ECL).
  • Overlay histogram showing HeLa cells stained with ab12450 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12450, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-FANCD2 antibody [103] (ab12450) 使用論文

This product has been referenced in:
  • Lyakhovich A & Surralles J FANCD2 depletion sensitizes cancer cells repopulation ability in vitro. Cancer Lett 256:186-95 (2007). ICC/IF ; Human . Read more (PubMed: 17643815) »

See 1 Publication for this product

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