製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to FANCC
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human, Recombinant fragment
  • 免疫原

    Recombinant fragment: MAQDSVDLSC DYQFWMQKLS VWDQASTLET QQDTCLHVAQ FQEFLRKMYE ALKEMDSNTV IERFPTIGQL LAKACWNPFI LAYDESQKIL IWCLCCLINK , corresponding to amino acids 1-101 of Human FANCC

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 発生段階
    Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
  • 細胞内局在
    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA80I15.1 antibody
    • FA 3 antibody
    • FA3 antibody
    • FAC antibody
    • FACC antibody
    • FANCC antibody
    • FANCC_HUMAN antibody
    • Fanconi anemia complementation group C antibody
    • Fanconi anemia complementation group C protein antibody
    • Fanconi anemia group C protein antibody
    • Fanconi pancytopenia type 3 antibody
    • FLJ14675 antibody
    • Protein FACC antibody
    see all

画像

  • Anti-FANCC antibody (ab54631) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FANCC antibody (ab54631) at 2 µg/ml

    Lane 1 : whole cell lysate prepared from human fibroblasts
    Lane 2 : whole cell lysate prepared from human fibroblasts
    Lane 3 : whole cell lysate prepared from human fibroblasts
    Lane 4 : whole cell lysate prepared from human fibroblasts

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP conjugated sheep anti-mouse IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa


    Exposure time : 3 minutes

    Image courtesy of Daniel Meier by Abreview

    See Abreview

  • ICC/IF image of ab54631 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54631, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab54631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

ab54631 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris, MES Buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Lukas Rambousek

Verified customer

投稿 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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