製品の概要

  • 製品名Anti-FANCA antibody
    FANCA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FANCA
  • 特異性Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    SRSYDHSENSDLVFG-C

    conjugated to KLH, corresponding to amino acids 995 - 1009 of Human FANCA.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa).

ターゲット情報

  • 機能DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • 関連疾患Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
  • 細胞内局在Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FA 1 antibody
    • FA antibody
    • FA H antibody
    • FA1 antibody
    • FAA antibody
    • FACA antibody
    • FAH antibody
    • Fanca antibody
    • FANCA_HUMAN antibody
    • FANCH antibody
    • Fanconi anemia complementation group A antibody
    • Fanconi anemia complementation group H antibody
    • Fanconi anemia group A protein antibody
    • Fanconi anemia type 1 antibody
    • MGC75158 antibody
    • Protein FACA antibody
    see all

Anti-FANCA antibody 画像

  • Anti-FANCA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µg

    Secondary
    IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 163 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Anti-FANCA antibody (ab5063) 使用論文

This product has been referenced in:
  • Vanuytsel K  et al. FANCA knockout in human embryonic stem cells causes a severe growth disadvantage. Stem Cell Res 13:240-50 (2014). WB . Read more (PubMed: 25108529) »
  • Shen C  et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). Read more (PubMed: 23633493) »

See all 7 Publications for this product

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP1 - Human acute monocytic leukemia cell)
Loading amount 150 µg
Specification THP1 - Human acute monocytic leukemia cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Wei-Ting Lu

Verified customer

投稿 Jul 24 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cal51 & SW620)
Loading amount 750 cells
Specification Cal51 & SW620
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Richard Allum

Verified customer

投稿 Oct 24 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"