製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FAN1
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human MTMR15, NP_055782.3

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
  • 配列類似性
    Belongs to the FAN1 family.
    Contains 1 UBZ-type zinc finger.
    Contains 1 VRR-NUC domain.
  • ドメイン
    The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
  • 細胞内局在
    Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6030441H18Rik antibody
    • Coiled coil domain containing protein MTMR15 antibody
    • DKFZp451H236 antibody
    • DKFZp686K16147 antibody
    • FAN1 antibody
    • FAN1_HUMAN antibody
    • FANCD2 and FANCI associated nuclease 1 antibody
    • FANCD2/FANCI associated nuclease 1 antibody
    • FANCD2/FANCI-associated nuclease 1 antibody
    • Fanconi associated nuclease 1 antibody
    • Fanconi-associated nuclease 1 antibody
    • hFAN1 antibody
    • KIAA1018 antibody
    • KMIN antibody
    • MTMR 15 antibody
    • MTMR15 antibody
    • Myotubularin related protein 15 antibody
    • Myotubularin-related protein 15 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FAN1 with ab95171 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-FAN1 antibody (ab95171) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 114 kDa
  • 3µg ab95171 were used to immunoprecipitate MTMR15 from 1mg HeLa whole cell lysate.
    20% IP was loaded and probed with ab95171 at 1µg/ml.
    Detection: chemiluminescence with exposure time of 10 seconds.

参考文献

ab95171 has not yet been referenced specifically in any publications.

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