製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FAM83G
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: RLLPDPGSPR LAQNARPMTD GRATEEHPSP FGIPYSKLSQ SKHLKARTGG SQWASSDSKR RAQ, corresponding to amino acids 753-815 of Human FAM83G

  • ポジティブ・コントロール
    • RT4, U-251 MG, Human Liver and Human Tonsil lysates, Human oral mucosa tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

ターゲット情報

画像

  • Immunocytochemistry/Immunofluorescence analysis of U-251 MG cells labelling FAM83G with ab121750 shows positivity in the cytoplasm.

  • All lanes : Anti-FAM83G antibody (ab121750) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique10-20% SDS PAGE
  • ab121750 at 1/100 dilution staining FAM83G in Paraffin-emebedded Human oral mucosa tissue by Immunohistochemistry.

参考文献

ab121750 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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