Anti-FAM83B 抗体 (ab122175)
Key features and details
- Rabbit polyclonal to FAM83B
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FAM83B antibody
FAM83B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FAM83B -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to amino acids 515-606 (ALGDRFEGYD NPENLKANAL YTHSRLRSSL VFKPTLPEQK EVNSCTTGSS NSTIIGSQGS ETPKEVPDTP TNVQHLTDKP LPESIPKLPL QS) of Human FAM83B.
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ポジティブ・コントロール
- Human duodenum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122175の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the FAM83 family. - Information by UniProt
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参照データベース
- Entrez Gene: 222584 Human
- SwissProt: Q5T0W9 Human
- Unigene: 657974 Human
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別名
- C6orf143 antibody
- FA83B_HUMAN antibody
- FAM83B antibody
see all
画像
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Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122175 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122175 at 1/200 dilution staining FAM83B in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab122175 は 1 報の論文で使用されています。
- Lin Q et al. Knocking down FAM83B inhibits endometrial cancer cell proliferation and metastasis by silencing the PI3K/AKT/mTOR pathway. Biomed Pharmacother 115:108939 (2019). PubMed: 31079003