製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FAM65A
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 849-965 of Human FAM65A

  • ポジティブ・コントロール
    • Human liver tissue, Human liver lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0

ターゲット情報

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  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121240 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-FAM65A antibody (ab121240) at 1/250 dilution

    Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Huam tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human liver tissue, using 1/35 ab121240, shows strong nuclear and cytoplasmic positivity in hepatocytes.

参考文献

ab121240 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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